Parallel assessment of CpG methylation by two-color hybridization with oligonucleotide arrays
نویسندگان
چکیده
منابع مشابه
Two color hybridization analysis using high density oligonucleotide arrays and energy transfer dyes.
High density oligonucleotide arrays (DNA chips) have been used in two color mutational analysis of the 3.43 kb exon 11 of the hereditary breast and ovarian cancer gene BRCA1 . Two color analysis allows competitive hybridization between a reference standard and an unknown sample, improving the performance of the assay. Fluorescein and phycoerythrin dyes werepreviously used due to their compatibi...
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A simple and rapid method for the analysis of genetic polymorphisms has been developed using allele-specific oligonucleotide arrays bound to glass supports. Allele-specific oligonucleotides are covalently immobilized on glass slides in arrays of 3 mm spots. Genomic DNA is amplified by PCR using one fluorescently tagged primer oligonucleotide and one biotinylated primer oligonucleotide. The two ...
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Methylation of CpG islands associated with genes can affect the expression of the proximal gene, and methylation of non-associated CpG islands correlates to genomic instability. This epigenetic modification has been shown to be important in many pathologies, from development and disease to cancer. We report the development of a novel high-resolution microarray that detects the methylation statu...
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The increasing interest in methylation patterns of mammalian DNA demands a simple and reliable method to clearly differentiate between cytosine (C) and 5-methylcytosine (5-MeC) within a specific DNA region. The simplest and most commonly used approach is the analysis with restriction endonucleases exhibiting different methylation sensitivities, like the MspI/HpaII pair of isoschizomeric enzymes...
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Multi-color patterning by polymer pen lithography (PPL) was used to fabricate covalently immobilized fluorophore and oligonucleotide arrays with up to five different components. The oligonucleotide arrays offer a virtually unlimited inventory of orthogonal binding tags for self-assembly of proteins as demonstrated by use of the arrays to monitor cell-protein interactions of MCF7 cells.
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2002
ISSN: 0003-2697
DOI: 10.1016/s0003-2697(02)00294-4